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Longlong
began his research assistantship in July, 1996. He is investigating
the higher level phylogenetic relationships of the Therevidae
using nucleotide sequence data in Brian Wiegmann's lab at
NCSU. He has successfully extracted
DNA from several genera and used PCR (polymerase chain
reaction) to amplify nuclear and mitochondrial genes for preliminary
nucleotide sequence comparisons at the genus level.
Mr.
Yang accompanied Mike Irwin, Don Webb, and the other four
graduate student dipterists to Guatemala
in May 1997. Upon returning to the US, he joined the other
students for a week in Illinois at the Irwin lab. While there,
all participants reported on their progress on the therevid
PEET project. Dr. Irwin presented the phylogenetic relationship
of the family Therevidae at subfamily and tribe levels based
on morphology. Mr. Shaun Winterton introduced his research
on dissection of the internal structure of female terminalia
and their phylogenetic significance. Mr. Yang demonstrated
the primary phylogenetic relationship of 20 therevid genera
and two outgroups using nucleotides of approximately 300 base
pairs in length of 28S rDNA. In addition, he labeled all the
individuals that were collected by in Guatemala and kept in
95% ethanol for molecular studies. A total of 36 individuals
belonging to four genera were identified. Collection records
were also input in therevid databases.
Yang
also traveled to meetings of the North American Dipterists
Society and the Entomological Society of America in 1997.
He prepared poster presentations for the NCSU Entomology departmental
retreat and the NSF PEET meeting in Woods Hole, MA in 1998.
He entered the student paper competition for the Presidents
Prize in the Entomological Society of America at the 1999
meeting in Atlanta Georgia, with his talk "Phylogenetics
of the stiletto flies (Diptera: Therevidae): Combined evidence
from gene sequences and morphology. His Ph.D. thesis entitled:
Higher-level Molecular Systematics of the Family Therevidae
(Diptera) is nearing completion.
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